HIV-1 Nef (full length) Antikörper

Produktnummer ABIN2452025

Der Meerschweinchen Polyklonal Anti- Antikörper wurde für WB, ELISA, DB und IP validiert. Er ist geeignet, in Proben von Human Immunodeficiency Virus (HIV) zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für HIV-1 Nef (full length) Antikörper (ABIN2452025)

Target: HIV-1 Nef

Reaktivität: Human Immunodeficiency Virus (HIV)

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB), Immunoprecipitation (IP)

Produktdetails

Bindungsspezifität: full length

Produktmerkmale: Whole Antiserum

Aufreinigung: Guinea Pig serum

Immunogen: Purified full-size recombinant Nef of HIV-1 subtype B expressed in E. coli

Anwendungsinformationen

Applikationshinweise: 1. Western blot: 1/1,000~1/3,000
2. Dot blot: 1/3000
3. Immunoprecipitation (assay dependent)
4. ELISA (assay dependent)
Other applications have not been tested

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Upon receipt aliquot and store at -20 C

Referenzen

Arora, Fredericksen, Garcia: "Nef: agent of cell subversion." in: Microbes and infection / Institut Pasteur, Vol. 4, Issue 2, pp. 189-99, (2002) (PubMed).

Fackler, Baur: "Live and let die: Nef functions beyond HIV replication." in: Immunity, Vol. 16, Issue 4, pp. 493-7, (2002) (PubMed).

Antigendetails

Target: HIV-1 Nef

Substanzklasse: Viral Protein

Hintergrund: HIV-1 Nef (27 kDa) is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection.